By J Tramper; et al
The foreign symposium ''Fundamentals of Biocatalysis in Non-Conventional Media'' used to be equipped less than auspices of the operating celebration utilized Biocatalysis of the ecu Federation of Biotechnology. one of the issues mentioned on the symposium have been physical-chemical facets reminiscent of pH, water-activity, viscosity, dielectric constants, polarity and so forth. relating to biocatalysis in non-conventional media. New measuring thoughts have been brought. for individuals operating within the box of biocatalysis in non-conventional media this ebook will provide a very good evaluate of the achieve in figuring out during the last 5 years of the basic elements of biocatalysis in non-conventional media
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Extra info for Biocatalysis in non-conventional media : proceedings of an international symposium, Noordwijkerhout, 26-29 April 1992
Hailing, Biochim. Biophys. Acta, 1 0 4 0 (1990) 2 2 5 - 2 2 8 . E . Wehtje, P. Adlercreutz and B . Mattiasson, (1992) (submitted). E . Wehtje, P. Adlercreutz and B . Mattiasson, (1992) this volume. H. Goderis, G. Ampe, M. Feyton, B . Fouwe, W. Guffens, S. Van Cauwenbergh and P. Tobback, Biotechnol. , 3 0 (1987) 2 5 8 - 2 6 6 . Biocatalysis in Non-Conventional Media, edited by J. Tramper et al. V. All rights reserved. 63 E N Z Y M E DESIGN FOR NONAQUEOUS MEDIA Jonathan S. Dordick, Zu-Feng X u , and Pramod Wangikar Department of Chemical and Biochemical Engineering, University of Iowa, Iowa City, IA 5 2 2 4 2 , USA Protein engineering and kinetic prediction by linear free energy relationships are tools that can be used to rationally optimize enzyme function in organic solvents.
When different a m o u n t s of water were added to a-chymotrypsin on hexyl- or glucose-CPG, the esterification activity was higher on the hexyl-CPG, regardless of the a m o u n t of water added. Obviously, there is also some other positive effect of the low-aquaphilicity supports. This will be discussed further in section 3 . 2 . 5 Figure 2 . The reaction rate obtained with a-chymotrypsin (O) and with h o r s e liver alcohol d e h y d r o g e n a s e (+) w h e n deposited on s u p p o r t materials with different aquaphilicity (Aq).
6. REFERENCES 1 2 3 4 5 6 7 8 T. Omata, T. Iida, A. Tanaka and S. Fukui, E u r . J . Appl. Microbiol. , 8 (1979) 1 4 3 - 1 5 5 . P. J . Hailing, Enzyme Microb. , 6 (1984) 5 1 3 - 5 1 6 . P. Adlercreutz, E u r . J . , 1 9 9 (1991) 6 0 9 - 6 1 4 . M. Reslow, P. Adlercreutz and B . Mattiasson, E u r . J . , 1 7 2 (1988) 5 7 3 - 5 7 8 . P. Hailing, Biochim. Biophys. Acta, 1 0 4 0 (1990) 2 2 5 - 2 2 8 . E . Wehtje, P. Adlercreutz and B . Mattiasson, (1992) (submitted). E . Wehtje, P. Adlercreutz and B .