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Download Carcinogenesis: Fundamental Mechanisms and Environmental by D. M. Jerina, J. M. Sayer, D. R. Thakker (auth.), Bernard PDF

By D. M. Jerina, J. M. Sayer, D. R. Thakker (auth.), Bernard Pullman, Paul O. P. Ts’o, Harry Gelboin (eds.)

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Read Online or Download Carcinogenesis: Fundamental Mechanisms and Environmental Effects: Proceedings of the Thirteenth Jerusalem Symposium on Quantum Chemistry and Biochemistry Held in Jerusalem, Israel, April 28 – May 2, 1980 PDF

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Additional info for Carcinogenesis: Fundamental Mechanisms and Environmental Effects: Proceedings of the Thirteenth Jerusalem Symposium on Quantum Chemistry and Biochemistry Held in Jerusalem, Israel, April 28 – May 2, 1980

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9 are shown the profiles of the product DNA obtained on neutral sucrose gradient centrifugation analysis. lg/ml of BPDE for 26 hours were 75% and 25%, respectively, of the control level. In contrast, when infected cells were pulsed with tritium labelled thymidine 2 hours after BPDE treatment, the inhibition of DN A synthesis was reduced to 15% of the level for untreated cells. Evidently, substantial recovery of the capacity for DNA synthesis takes place between 2 and 26 hours following treatment.

The ESR signal of the free radical formed by incubating anthanthrene or 10-aza-B[ajP with rat liver microsomes (Me-induced) and the signal of the covalently bound adduct of the radical with poly:G). Reaction condition and sample preparation are given in ref. 7. 46 C. NAGATA ET AL. 3. 7,12-Dimethylbenz[a)anthracene (DMBA) Carcinogenicity of DMBA is known to be the most potent among aromatic hydrocarbon carcinogens. Nonetheless, the metabolic pathways of this compound have not been extensively studied.

P. Ts'o and H. ), Carcinogenesis: Fundamental Mechanisms and Environmental Effects, 43-54. Copyright © 1980 by D. Reidel Publishing Company. 44 C. NAGATA ET AL. forming the free radical in microsome system (Table i). Twelve hydroxy B[a]P can be classified into three groups. Group 1: NADPH is required for the free radical formation and 2-, 4-, 9-, and ll-OH derivatives belong to this group. Group 2: No ESR signal was detected in the presence and absence of NADPH and 3- and 12-0H derivatives belong to this group.

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