By Burr G. Atkinson
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Tissières, Α . , Schedl, P . , and Gehring, W. J. (1979). Physical map of two Drosophila melanogaster DNA segments containing sequences coding for the 70,000 dalton heat shock protein. Cell 17, 1-8. Neidhardt, F. C , and Van Bogelen, R. A. (1981). Positive regulatory gene for temperature-controlled proteins in Escherichia coli. Biochem. Biophys. Res. Commun. 100, 8 9 4 - 9 0 0 . 1. Gene Organization, Sequences, and Induction 29 Neidhardt, F. C , Van Bogelen, R. , and Lau, Ε. T. (1983). Molecular cloning and expression of a gene that controls the high temperature regulon of Escherichia coli.
Labeled DNA was incubated with puff-inducing protein (Fig. 2% agarose gel. Lanes: 1, DNA starting material; 2, DNA bound by undiluted protein sample; 3 , DNA bound by 1:135 dilution; 4, DNA bound by 1:1215 dilution; 5, no protein control. The 2100-base fragment exhibits much more significant binding than do the other fragments. (B) The hsp70 clone used here is a BamUl subclone from a tandem repeat of hsp70 genes. Thin lines, pBR322; boxes, hsp70 gene; arrows, direction of transcription of this "circularly permuted" gene; knob on right-hand arrow = 5' end; cross hatches, Pstl sites.
Genetics 92, 8 9 1 - 9 0 2 . Ritossa, F. M. (1962). A new puffing pattern induced by heat shock and DNP in Drosophila. Experientia 18, 5 7 1 - 5 7 5 . Ritossa, F. M. (1964a). Experimental activation of specific loci in polytene chromosomes of Drosophila. Exp. Cell Res. 35, 6 0 1 - 6 0 7 . Ritossa, F. M. (1964b). Behaviour of RNA and DNA synthesis at the puff level in salivary gland chromosomes of Drosophila. Exp. Cell. Res. 36, 5 1 5 - 5 2 3 . Rubin, G. M . , and Spradling, A. C. (1982). Genetic transformation of Drosophila with transposable element vectors.