Industrial Technical

Download Downstream Processing by J.A. Wesselingh, J. Krijgsman PDF

By J.A. Wesselingh, J. Krijgsman

Keeping apart and purifying the specified product is a crucial a part of any bio-project. This processing ‘downstream’ from the fermenter is either tough and costly. This e-book introduces all of the universal strategies, beginning within the lab and finishing within the plant. now we have written it for undergraduates in procedure engineering (biotechnological, mechanical or chemical) and graduate microbiologists doing a minor at the expertise that's required to scale up their experiments to a advertisement scale

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Extra resources for Downstream Processing

Example text

The strength and density of a floc have been shown to be directly related quantities 93). Optimum strength of soya protein precipitate aggregates is achieved when the product of shear rate and ageing time exceeds 105 (Fig. 15). See Sects. 4 and 5 for further discussion of the precipitate properties. Theoretical or even empirical models for the prediction of shear break-up of protein precipitates have not proved successful due to factors such as the timedependent properties of the precipitate during shear break-up.

14. Effect of rate of shear on is9 soya protein precipitate particle size 89) Time of shearing, 300 s ( 9 1800 s ( o ) . Ranges of dg0 values for times of shearing from 300 s to 7200 s are shown. 5 % weight; temperature, 23 ~ 001 ................. n i , i i . . 104 105 the rate of orthokinetic aggregation during which large precipitate particles mop up the fines. A most important factor determining protein precipitate aggregate strength is the history of preparation. Unless the precipitate is exposed to sufficient deformation forces it will not develop a compact structure.

Detailed studies of the effect of the theological characteristics on centrifuge performance are rare, although there is extensive literature on the rheology of both dilute 16~) and concentrated dispersions 162) with considerable advances being made in the theoretical description of dispersion flow properties 163,164,165,~66). Many protein precipitates will not dewater readily, resulting in wet sludges which are contaminated with significant quantities of supernatant and so require a series of washing steps which contribute to product loss through low efficiency and denaturation.

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